LC3 monoclonal antibody (5H3)
Product Specifications
Product Name Alternative
Microtubule-associated protein 1 light chain 3, MAP1LC3
Certification
RUO
UniProt
Q9H492 (LC3A), Q9GZQ8 (LC3B), Q9BXW4 (LC3C)
Host
Mouse
Species Reactivity
Human
Immunogen
Synthetic peptide corresponding to an internal sequence identical in LC3A, LC3B and LC3C conjugated to hemocyanin.
Clone
5H3
Applications
ICC, IHC, WB
Purification
Thiophilic adsorption and size exclusion chromatography purified.
Positive Control
Included. (Prod. No. ALX-840-038)
Stability
Stable at -20°C up to 1 year.
Dilution
Immunocytochemistry (paraformaldehyde/methanol fixation; 1-10 μg/ml) Western Blot (0.5μg/ml for HRPO/ECL detection; Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) We strongly recommend to use PVDF membranes for immunoblot assays.Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Specificity
Recognizes both forms of endogenous LC3.
Formulation
Liquid. In PBS containing 50% glycerol, 0.09% sodium azide, PEG and sucrose.
Applications Notes
Detects a band of ~18kDa (LC3-I; cytoplasmic form) and a band of ~16kDa (LC3-II; lipidated form) by Western blot.
Warranty
This antibody is covered by our Worry-Free Guarantee .
Isotype
IgG1
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