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KillerTRAILTM Protein (soluble) (human), (recombinant)

Product Specifications

Specifications

ED 50 : 20ng/ml (BJAB cells)

Product Name Alternative

Apo-2L, TNFSF 10, CD253

Certification

RUO

UniProt

P50591

Source

Produced in E. coli . The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide.

Purification

Affinity purified. Sterile filtered.

Stability

Stable for at least 12 months after receipt when stored at -80° C.

Endotoxin

≤0.01 EU/μg purified protein (LAL test) .

Purity

≥99% (SDS-PAGE, MS analysis)

Activity

Induces apoptosis in a concentration range of 10-100ng/ml. Note: Does not require a cross-linking enhancer for its potent biological activity. For cell lines that require extensive cross-linking of the TRAIL-Rs for killing (e.g. Jurkat) use SUPERKILLERTRAIL ® (Prod. No. ALX-201-115) .

Reconstitution

For a special storage and dilution buffer see KillerTRAILTM Storage and Dilution Buffer (Prod. No. ALX-505-005) .

Molecular Weight

~24kDa (SDS-PAGE)

Shipping Conditions

Dry Ice

Storage Conditions

-80°C

Specificity

Binds to human and mouse TRAIL receptors and osteoprotegerin (OPG) .

Formulation

Liquid. HEPES, pH 7.4, sodium chloride, Tween 20, sucrose, azide free and 1mM DTT.

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