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IL-6 (mouse), (recombinant)

Product Specifications

Product Name Alternative

Interleukin-6, IFN-β2, B-cell differentiation factor, BSF-2, HSF, MGI-2

Certification

RUO

UniProt

P08505

Source

Produced in E. coli . Non-glycosylated protein, containing 187 amino acids.

Stability

It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.

Endotoxin

≤1 EU/μg protein measured by kinetic LAL analysis.

Purity

≥95% (HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm)

Activity

The activity is determined by dose-dependent induction of 7TD1 cell proliferation which is typically observed at concentations <0.02ng/ml.

Reconstitution

When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized mouse IL-6 in 10 mM HCl to 0.1-1.0 mg/ml, which can be further diluted to other aqueous solutions.

Molecular Weight

~21.7kDa

Shipping Conditions

Ambient Temperature

Storage Conditions

-20°C

Appearance

White lyophilized (freeze-dried) powder.

Formulation

Lyophilized. Sterile filtered.

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