IL-35 (human) :Fc (human), (recombinant)
Product Specifications
Product Name Alternative
Interleukin-35, EBI3:IL-12A, IL-27B:CLMF p35
Certification
RUO
UniProt
Q14213 (IL-27B), P29459 (IL-21A)
Source
Produced in HEK 293 cells. Human EBI3 (aa 21-229) linked to human IL-12A (aa 23-219) via the linker GGGSGGGSGGGSGGGS is fused at the C-terminus to the Fc portion of human IgG1 and a FLAG ® -tag.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
0.1 mg/mL after reconstitution.
Endotoxin
<0.1EU/μg purified protein (LAL test; Associates of Cape Cod) .
Purity
≥95% (SDS-PAGE)
Reconstitution
Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Molecular Weight
~85kDa
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
Historical data has shown that IL-35 binds to IL-12Rß2, and it has been demonstrated in the literature to inhibit mononuclear cell proliferation. FLAG is a registered trademark of Sigma-Aldrich Co.
Formulation
Lyophilized. Contains PBS.
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