Hepsin (human), (recombinant)
Product Specifications
Specifications
~2.5U/μl. One unit is defined at the amount of enzyme that releases 1nmol of AMC per min. at 37°C in the presence of 50μM Boc-Gln-Ala-Arg-AMC.
Product Name Alternative
Transmembrane protease serine 1, TMPRSS1
Certification
RUO
UniProt
P05981
Source
Produced in Drosophila Schneider 2 (S2) cells. Recombinant human hepsin (aa 45-417) is fused at the C-terminus to a V5-His-tag. Produced by plasmid transfection expression.
Stability
Stable for at least 6 months when stored at -20°C. Recommended Assay Buffer: 100mM TRIS, pH 9.0, containing 5mg/ml BSA. Do not store diluted solutions.
Concentration
100nM
Activity
Trypsin-like serine protease.
Molecular Weight
~45kDa; a-subunit: ~15kDa; b-subunit: ~30kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 100mM TRIS, pH 9.0, containing 200mM sodium chloride and 10% glycerol.
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