HDAC8 (human), (recombinant)

≥0.4 U/μg. One unit is defined as the amount of enzyme that deacetylates 1pmol/min of FLUOR DE LYS® deacetylase substrate (Prod. No. BML-KI104) at 37°C /250μM.

The enzyme is stable on ice for the time typically required to set up an experiment (30-60 min.), but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay Wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best results will be obtained by adding the chilled, undiluted enzyme directly to prewarmed buffer and proceeding immediately to the addition of pre-warmed substrate. Either add a small volume of undiluted enzyme to each well (e.g. 1 U per well) or dilute into pre-warmed buffer (e.g. 1 U per 25 μl) and immediately aliquot to Wells (see attached assay conditions) . The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC8 into separate tubes and store at -70°C.NOTE: When stored under the above conditions, this enzyme is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution of the enzyme followed by refreezing, could lead to loss of activity.

Useful tool to study HDAC8 kinetics and inhibitor sensitivity and also the effect of the enzyme in transcriptional regulation, cell cycle progression, and oncogenesis.