HDAC2 (full-length) (human), (recombinant) (His-tag)
Product Specifications
Specifications
≥0.5 U/μg. One U=1 pmol/min at 37°C, 100 μM, FLUOR DE LYS ® -SIRT1 substrate (p53 K (Ac) -382 fluorogenic substrate, Prod. No. BML-KI177) .
Product Name Alternative
Histone deacetylase 2
Certification
RUO
UniProt
Q92769
Source
Produced in insect cells. Produced in a baculovirus expression system.
Sequence
HDAC2 from human cDNA. Sequence is identical to residues 1-582 at Genbank accession# NM_00157.2.
Purification
Partially purified by single-step affinity chromatography and gel filtration.
Stability
The enzyme is stable on ice for the time typically required to set up an experiment (30-60 min.), but may lose activity with prolonged storage on ice. It is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay. The remaining, unused, undiluted enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. Freezing and storage of diluted enzyme is not recommended.
Molecular Weight
~66 kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 50mM TRIS, pH 8.0, 138 mM NaCl and 10% glycerol.
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