Growth and differentiation factor-5 (human), (recombinant)
Product Specifications
Product Name Alternative
GDF-5, CDMP-1, BMP-14, Bone morphogenetic protein 14, Cartilage-derived morphogenetic protein 1, LAP-4, LPS-associated protein 4, Radotermin
Certification
RUO
UniProt
P43026
Source
Produced in E. coli . Non-glycosylated homodimer, containing two 117 amino acids chains. To enable bacterial expression the N-terminal sequence of Ala-Pro-Leu-Thr was replaced with a Lys.
Stability
It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Endotoxin
≤1 EU/μg protein measured by kinetic LAL analysis.
Purity
≥98% (HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm)
Activity
The activity is determined by the ability to induce alkaline phosphatase activity in ATDC5 cells which is typically observed at a concentration of 0.1-1μg/ml.
Reconstitution
When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Molecular Weight
~28.8kDa
Shipping Conditions
Ambient Temperature
Storage Conditions
-20°C
Appearance
White lyophilized (freeze-dried) powder.
Formulation
Lyophilized. Sterile filtered.
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