Growth and differentiation factor-15 (human), (recombinant)
Product Specifications
Product Name Alternative
GDF-15, MIC-1, Placental TGF-β, Prostate differentiation factor
Certification
RUO
UniProt
Q99988
Source
Produced in E. coli . Non-glycosylated, disulfide linked homodimer, containing two identical 113 amino acid chains.
Stability
It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Endotoxin
≤1 EU/μg protein measured by kinetic LAL analysis.
Purity
≥95% (HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm)
Activity
The activity is determined by the inhibition of DU-145 cells which is typically observed at a concentration of 1-2μg/ml.
Reconstitution
When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 5 mM acetic acid (AcOH) at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Molecular Weight
~24.5kDa
Shipping Conditions
Ambient Temperature
Storage Conditions
-20°C
Appearance
White lyophilized (freeze-dried) powder.
Formulation
Lyophilized. Sterile filtered.
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