GOLGI ID® Green assay kit

With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.

The GOLGI ID ® Green assay kit is a member of the CELLESTIAL ® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL ® reagents and kits are optimal for use in demanding cell analysis applications involving confocal microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required.

The GOLGI ID ® Green assay kit is specifically designed for use in the fluorescence co-localization imaging of red fluorescent protein (RFP) -expressing cell lines as well as cells expressing blue, cyan or orange fluorescent proteins (BFPs, CFPs, OFPs), a powerful approach for determining the targeting of molecules to intracellular compartments and for screening of their associations and interactions. The kit contains a Golgi apparatus-selective dye suitable for live cell, or aldehyde-fixed cell staining.

A sample from each lot of GOLGI ID ® Green assay kit is used to stain HeLa cells, using the procedures described in the user manual. The selectivities of the GOLGI ID ® Green dye and the Hoechst 33342 dye are evident.