FLUOR DE LYS®-Green HDAC fluorometric activity assay kit

Nuclear Extract from HeLa Cells (human cervical cancer cell line) (Prod. No. BML-KI140) (100μl in 0.1M potassium chloride, 20mM HEPES/NaOH, 20% (v/v) glycerol, 0.2mM EDTA, 0.5mM DTT, 0.5mM PMSF) Storage: -70°C. FLUOR DE LYS ® -Green Substrate (Prod. No. BML-KI572) (50μl 50mM in DMSO) Storage: -70°C. FLUOR DE LYS ® Developer Concentrate (20X) (Prod. No. BML-KI105) (300μl 20x stock solution; dilute in assay buffer before use) Storage: -70°C. Trichostatin A (Prod. No. BML-GR309) (100μl 0.2mM in DMSO) Storage: -70°C. FLUOR DE LYS ® -Green Standard (Prod. No. BML-KI605) (30μl 1mM in DMSO) Storage: -70°C. Note: BML-KI605 is an improved replacement of BML-KI573. NAD + (Sirtuin Substrate) (Prod. No. BML-KI282) (500μl; 50mM in 50mM TRIS, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride) Storage: -70°C. Nicotinamide (Prod. No. BML-KI283) (500μl; 50mM in 50mM TRIS, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride) Storage: -70°C. HDAC Assay Buffer (Prod. No. BML-KI143) (20ml; 50mM TRIS, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride) Storage: -70°C. 96 Well Plates (Prod. No. BML-KI101) Storage: Room temperature White NBS Microplate (Prod. No. BML-KI571) Storage: Room temperature