FLUOR DE LYS®HDAC3/NCOR1 fluorometric drug discovery kit

Store all components, except the microplate, at -80°C for the highest stability. The HDAC3/NCOR1 Complex, Prod. No. BML-KI574, must be handled with particular care in order to retain maximum enzymatic activity. Defrost it quickly in a RT water bath or by rubbing between fingers, then immediately store on an ice bath. The remaining unused enzyme should be refrozen quickly, by placing at -80°C. If possible, snap freeze in liquid nitrogen or a dry ice/ethanol bath. To minimize the number of freeze/thaw cycles, aliquot the enzyme into separate tubes and store at -80°C.

HDAC3/NCOR1 Complex (human, recombinant) (Prod. No. BML-KI574) 67 μl; 30 ng/μl in 50mM TRIS, pH 8.0, 138mM NaCl, 10% glycerol, 1 mg/ml BSA. Storage: -80°C; avoid freeze/thaw cycles! FLUOR DE LYS ® −SIRT1, Deacetylase Substrate (Prod. No. BML-KI177) 100 μl; 5mM solution in 50mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride. Storage: -80°C FLUOR DE LYS ® Developer II Concentrate (5x) (Prod. No. BML-KI176) 5 x 250 μL; 5x Stock Solution; dilute in assay buffer before use. Storage: -80°C Trichostatin A (HDAC Inhibitor) (Prod. No. BML-GR309-9090) 100 μl; 0.2mM in DMSO Storage: -80°C FLUOR DE LYS ® Deacetylated Standard (Prod. No. BML-KI142) 30μl; 10mM in DMSO Storage: -80°C HDAC Assay Buffer II (Prod. No. BML-KI422) 20 ml; (50 mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride, 1mg/ml BSA) . Storage: -20°C HDAC Assay Buffer (Prod. No. BML-KI143) 20 ml; (50 mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride) . Storage: -20°C 1/2 volume microplate (Prod. No. BML-KI101) Storage: Ambient 1/2 volume white NBS microplate (Prod. No. BML-KI571) Storage: Ambient