FLUOR DE LYS®HDAC1 fluorometric drug discovery assay kit

Store all components except the microplate and instruction booklet at -80°C for the highest stability. The HDAC1 Enzyme (Prod. No. BML-SE456), must be handled with particular care in order to retain maximum enzymatic activity. Defrost it quickly in a RT water bath or by rubbing between fingers, then immediately store on an ice bath. The remaining unused enzyme should be refrozen quickly, by placing at -80°C. If possible, snap freeze in liquid nitrogen or a dry ice/ethanol bath. To minimize the number of freeze/thaw cycles, aliquot the enzyme into separate tubes and store at -80°C.

HDAC1 (Histone Deacetylase 1) (human, recombinant) (Prod. No. (BML-SE456) (50 μg; 10mM TRIS, pH 7.5, 100mM sodium chloride, 3mM magnesium chloride, 10% glycerol) Storage: -80°C, avoid freeze/thaw cycles FLUOR DE LYS ® SIRT1, Deacetylase Substrate (Prod. No. BML-KI177) (100μl; 5mM solution in 50mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride) Storage: -80°C FLUOR DE LYS ® Developer II Concentrate (5x) (Prod. No. BML-KI176) (5 x 250 μL; 5x Stock Solution; Dilute in Assay Buffer before use) Storage: -80°C Trichostatin A (HDAC Inhibitor) (Prod. No. BML-GR-309-9090) (100 μl; 0.2mM in DMSO) Storage: -80°C FLUOR DE LYS ® Deacetylated Standard (Prod. No. BML-KI142) (30 μl; 10mM in DMSO) Storage: -80°C HDAC Assay Buffer II (50mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride, 1mg/ml bovine serum albumin) (Prod. No. BML-KI422) (20 ml) Storage: -20°C (or -80°C) HDAC Assay Buffer (50mM TRIS/Cl, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride) (Prod. No. BML-KI143) (20 ml) Storage: -20°C (or -80°C) 1/2 Volume Microplates (Prod. No. 80-2407) 1 clear and 1 white, 96-well Storage: Ambient