Fc (human) :TNF-α (soluble) (human), (recombinant)
Product Specifications
Product Name Alternative
Tumor necrosis factor α, TNFSF 2
Certification
RUO
UniProt
P01375
Source
Produced in CHO cells. The extracellular domain of human TNF-α (aa 85-233) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa) .
Purification
Purified by multi-step chromatography.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
0.1 mg/mL after reconstitution.
Endotoxin
<0.1EU/μg purified protein (LAL test; Associates of CAPE COD Inc.) .
Purity
≥90% (SDS-PAGE)
Reconstitution
Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Molecular Weight
~48kDa (calculated), ~52kDa (observed)
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
Historical data has shown that TNFα induces killing of WEHI-164 cells in a concentration range of 0.5-2ng/ml (does not require an enhancer) .
Formulation
Lyophilized. Contains PBS.
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