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Fc (human) :TNF-α (soluble) (human), (recombinant)

Product Specifications

Product Name Alternative

Tumor necrosis factor α, TNFSF 2

Certification

RUO

UniProt

P01375

Source

Produced in CHO cells. The extracellular domain of human TNF-α (aa 85-233) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa) .

Purification

Purified by multi-step chromatography.

Stability

Stable for at least 6 months after receipt when stored at -20°C.

Concentration

0.1 mg/mL after reconstitution.

Endotoxin

<0.1EU/μg purified protein (LAL test; Associates of CAPE COD Inc.) .

Purity

≥90% (SDS-PAGE)

Reconstitution

Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.

Molecular Weight

~48kDa (calculated), ~52kDa (observed)

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

Historical data has shown that TNFα induces killing of WEHI-164 cells in a concentration range of 0.5-2ng/ml (does not require an enhancer) .

Formulation

Lyophilized. Contains PBS.

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