Fc (human) :CD40L (soluble) (mouse), (recombinant)
Product Specifications
Product Name Alternative
CD154, TNFSF 5, gp39
Certification
RUO
UniProt
P27548
Source
Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa) .
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
0.1 mg/mL after reconstitution.
Endotoxin
<0.1EU/μg purified protein (LAL test; Associates of Cape Cod) .
Purity
≥90% (SDS-PAGE)
Reconstitution
Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Molecular Weight
~58kDa (SDS-PAGE) .
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
Historical data has shown that Fc (human) :CD40L (mouse) stimulates the proliferation of mouse B cells and dendritic cells. Note: Does not require an enhancer.
Specificity
Binds to human and mouse CD40.
Formulation
Lyophilized. Contains PBS.
Applications Notes
ELISA: binds to human CD40:COMP at 10-100 ng/ml.
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