FasL, soluble (human) (recombinant) set
Product Specifications
Specifications
ED 50 : 1ng/ml (A20 cells)
Product Name Alternative
APO-1L, CD95L, CD178, TNFSF 6
Certification
RUO
UniProt
P48023
Source
Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG ® -tag. Glycosylation of FasL, Soluble (human) (recombinant) is similar to natural human FasL.
Applications
FUNC
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
FasL: 0.1 mg/mL after reconstitutionEnhancer: 1 mg/mL
Purity
≥95% (SDS-PAGE)
Reconstitution
Reconstitute FasL with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
FLAG is a registered trademark of Sigma-Aldrich Co.
Specificity
FasL binds to human, mouse and rat Fas (CD95) .
Formulation
FasL: Lyophilized. Contains PBS. Enhancer: Liquid. In PBS, pH7.4, containing 50% glycerol. Contains no preservatives.
Applications Notes
Kills Fas-sensitive cells at concentrations of >1ng/ml in the presence of cross-linking enhancer.
Contents
10μg of FasL, Soluble (human) (recombinant) (Prod. No. ALX-522-001) and 4x50μg of Enhancer for Ligands (Prod. No. ALX-804-034) which increases the biological activity (induction of apoptosis) by ~50-fold to a concentration range of 1-5ng/ml.
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