FasL (soluble) (human), (recombinant)
Product Specifications
Product Name Alternative
APO-1L, CD95L, CD178, TNFSF 6
Certification
RUO
UniProt
P48023
Source
Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG ® -tag. Glycosylation of rhsFasL is similar to that of natural human FasL.
Purification
Purified by multi-step chromatography.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
0.1 mg/mL after reconstitution.
Endotoxin
<0.1EU/μg purified protein (LAL test; Associates of Cape Cod) .
Purity
≥95% (SDS-PAGE)
Activity
Kills Jurkat cells in the absence of a cross-linker. Cross-linking enhancer (see Set Prod. No. ALX-850-014) increases the activity of rhsFasL approx. 50-fold. Attention: Results using sFasL may differ from those obtained with agonistic antibodies!
Reconstitution
Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
Molecular Weight
~32kDa (non-glycosylated), ~35kDa (glycosylated) (SDS-PAGE) .
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
Historical lots have shown that FasL kills Jurkat cells at concentrations of >10ng/ml in the absence of a cross-linker. Historical lots have also shown an ED 50 of 50ng/ml (A20 cells) .
Specificity
Binds to human, mouse and rat Fas (CD95; APO-1) .
Formulation
Lyophilized. Contains PBS.
Applications Notes
ELISA: binds to Fas receptor at 1-100 ng/ml.
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