Fas (human) :Fc (human), (recombinant)
Product Specifications
Product Name Alternative
CD95, APO-1, TNFRSF 6
Certification
RUO
UniProt
P25445
Source
Produced in HEK 293 cells. The extracellular domain of human Fas (CD95; APO-1) (aa 7-154) is fused to the Fc portion of human IgG1.
Purification
Purified by multi-step chromatography.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
1 mg/mL after reconstitution.
Endotoxin
<0.1EU/μg purified protein (LAL test; Associates of Cape Cod) .
Purity
≥95% (SDS-PAGE)
Reconstitution
Reconstitute with 50μl sterile water. Further dilutions can be made with medium containing 5% fetal calf serum or a carrier protein.
Molecular Weight
~56 kDa
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
Historical data has shown that Fas inhibits the activity of human and mouse soluble Fas (sFasL) and thereby sFasL-mediated lysis of Fas sensitive cells in a concentration range of 20-100μg/ml. When used with cross-linking enhancer (see Prod. No. ALX-203-001), the inhibitory activity of rhFas:Fc is increased approximately 20- to 50-fold. Concentrations of rhFas:Fc required to inhibit may vary depending on the cell viability and on the concentrations of sFasL used to kill the cells.
Specificity
Binds human, mouse and rat FasL (APO-1L; CD95L; CD178) .
Formulation
Lyophilized. Contains PBS.
Applications Notes
ELISA: binds to FasL at 10-100 ng/ml.
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