Estrogen receptor α monoclonal antibody (33)
Product Specifications
Product Name Alternative
ERα
Certification
RUO
UniProt
P03372
Host
Mouse
Species Reactivity
Bovine,Human,Monkey,Mouse,Rat
Immunogen
Synthetic peptide corresponding to aa 247-261 (E247VGMMKGGIRKDRRG261) of the DNA binding domain of ERα (estrogen receptor α) . This sequence is completely conserved in many species including mouse, rat, pig, bovine, sheep, chicken and frog.
Clone
33
Applications
Flow Cytometry, ICC, IF, IHC (FS), IHC (PS), IP, WB
Dilution
Immunofluorescence (10μg/mL) Immunohistochemistry (1:20-1:100) Western Blot (4 μg/ml) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Specificity
Recognizes both the steroid occupied and unoccupied forms of ERα.
Formulation
Liquid. In Dulbecco’s PBS, containing 0.05% sodium azide, 1mg/mL BSA and 30% glycerol.
Applications Notes
Immunohistochemistry: Staining of ERα in mouse Leydig cells results in both nuclear and cytoplasmic staining. Detects a band of ~67kDa by Western blot. Does not inhibit binding of ER to nuclei and DNA.
Warranty
This antibody is covered by our Worry-Free Guarantee .
Isotype
IgG1
Cross Reactivity Details
Does not cross-react with androgen receptor, PR (progesterone receptor), GR (glucocorticoid receptor) or ERβ.
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