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Estrogen receptor α monoclonal antibody (33)

Product Specifications

Product Name Alternative

ERα

Certification

RUO

UniProt

P03372

Host

Mouse

Species Reactivity

Bovine,Human,Monkey,Mouse,Rat

Immunogen

Synthetic peptide corresponding to aa 247-261 (E247VGMMKGGIRKDRRG261) of the DNA binding domain of ERα (estrogen receptor α) . This sequence is completely conserved in many species including mouse, rat, pig, bovine, sheep, chicken and frog.

Clone

33

Applications

Flow Cytometry, ICC, IF, IHC (FS), IHC (PS), IP, WB

Dilution

Immunofluorescence (10μg/mL) Immunohistochemistry (1:20-1:100) Western Blot (4 μg/ml) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Specificity

Recognizes both the steroid occupied and unoccupied forms of ERα.

Formulation

Liquid. In Dulbecco’s PBS, containing 0.05% sodium azide, 1mg/mL BSA and 30% glycerol.

Applications Notes

Immunohistochemistry: Staining of ERα in mouse Leydig cells results in both nuclear and cytoplasmic staining. Detects a band of ~67kDa by Western blot. Does not inhibit binding of ER to nuclei and DNA.

Warranty

This antibody is covered by our Worry-Free Guarantee .

Isotype

IgG1

Cross Reactivity Details

Does not cross-react with androgen receptor, PR (progesterone receptor), GR (glucocorticoid receptor) or ERβ.

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