ERK1/2 monoclonal antibody (12D4)

Product Specifications

Product Name Alternative

MAPK2/3, MAP kinase 2/3

Certification

RUO

UniProt

P27361 (Erk1), P28482 (Erk2)

Host

Mouse

Species Reactivity

Dog,Human,Mouse,Rat

Immunogen

Peptide comprising the pThr-Glu-pTyr motif in activated ERK1 and ERK2 conjugated to KLH.

Clone

12D4

Applications

ELISA, ICC, IHC, IP, WB

Purification

Thiophilic adsorption and size exclusion chromatography purified.

Positive Control

Included. (Prod. No. ALX-840-025)

Stability

Stable at -80°C up to 1 year, at 4°C up to 3 months.

Dilution

Immunocytochemistry (1-10μg/ml) Immunoprecipitation (1-10μg per 106 pervanadate-treated A431 or HepG2 cells) Western Blot (0.5μg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Reconstitution

Reconstitute with 1ml water (15 minutes at room temperature) .

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

Replacement for ADI-905-651

Specificity

Recognizes the pThr-Glu-pTyr motif of activated ERK1 and ERK2. Requires phosphorylation both at the threonine and the tyrosine site and does not cross-react with the non-phosphorylated form of the protein.