Epidermal growth factor (rat), (recombinant)
Product Specifications
Product Name Alternative
EGF, Urogastrone, URG
Certification
RUO
UniProt
P07522
Source
Produced in E. coli . Non-glycosylated protein, containing 54 amino acids, with a molecular weight of 6.3 kDa.
Stability
It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
Endotoxin
≤1 EU/μg protein measured by kinetic LAL analysis.
Purity
≥95% (HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm)
Activity
The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells which is typically observed at concentations <0.1ng/ml.
Reconstitution
When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Molecular Weight
~6.3kDa
Shipping Conditions
Ambient Temperature
Storage Conditions
-20°C
Appearance
White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from 10mM Na 2 PO 4 , pH 7.5. Sterile filtered.
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