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Epidermal growth factor (rat), (recombinant)

Product Specifications

Product Name Alternative

EGF, Urogastrone, URG

Certification

RUO

UniProt

P07522

Source

Produced in E. coli . Non-glycosylated protein, containing 54 amino acids, with a molecular weight of 6.3 kDa.

Stability

It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.

Endotoxin

≤1 EU/μg protein measured by kinetic LAL analysis.

Purity

≥95% (HPLC, Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm)

Activity

The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells which is typically observed at concentations <0.1ng/ml.

Reconstitution

When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Molecular Weight

~6.3kDa

Shipping Conditions

Ambient Temperature

Storage Conditions

-20°C

Appearance

White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from 10mM Na 2 PO 4 , pH 7.5. Sterile filtered.

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