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EpCAM Recombinant monoclonal antibody (rMOC-31)

Product Specifications

Product Name Alternative

Epithelial cell adhesion molecule, Epithelial specific antigen, ESA, CD326

Certification

RUO

UniProt

P16422

Host

Mouse

Species Reactivity

Human

Immunogen

Neuraminidase treated GLS-1 human small cell lung carcinoma cells.

Clone

RMOC-31

Applications

Flow Cytometry, IF, IHC (PS), WB

Purification

Protein G affinity purified.

Dilution

Flow Cytometry (0.5-1 μg/106 cells in 0.1 mL) Immunofluorescence (1-2 μg/mL) Western blot (0.5-1 μg/mL) Immunohistochemistry (formalin-fixed paraffin sections) (0.5-1 μg/mL for 30 min at RT) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Shipping Conditions

Blue Ice

Storage Conditions

+4°C

Specificity

Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM.

Formulation

Liquid. In 1X PBS containing 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide.

Applications Notes

This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Warranty

This antibody is covered by our Worry-Free Guarantee .

Isotype

LgG1κ

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