ENOS (human), (recombinant)
Product Specifications
Specifications
≥120 U/mg. 1 U is defined as the amount of enzyme required to produce 1 nmol of NO (nitric oxide) per minute at 37°C. NO synthesis is measured by the conversion of oxyhemoglobin to methemoglobin.
Product Name Alternative
Nitric oxide synthase (endothelial), NOS III
Certification
RUO
UniProt
P29474
Source
Produced in Sf9 insect cells. Untagged human eNOS. Produced in a baculovirus expression system.
Stability
ENOS is relatively unstable, and loses activity on ice within 30 minutes. Prepare aliquots and snap-freeze in liquid nitrogen until ready for use.
Purity
≥90% (SDS-PAGE, Western Blot)
Molecular Weight
~135kDa/subunit; homodimer.
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 50 mM TRIS, pH 7.4, containing 0.1mM EDTA, 10% glycerol, 100mM NaCl, 0.1mM DTT, 10 μM tetrahydrobiopterin.
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