EGF receptor (human) monoclonal antibody (13G8)
Product Specifications
Product Name Alternative
Epidermal growth factor receptor, EGFR, HER-1
Certification
RUO
UniProt
P00533
Host
Mouse
Species Reactivity
Human,Mouse
Immunogen
Synthetic peptide corresponding to aa 1165-1186 of EGF receptor (epidermal growth factor receptor) conjugated to KLH.
Clone
13G8
Applications
ELISA, ICC, IP, Luminex, WB
Purification
Thiophilic adsorption and size exclusion chromatography purified.
Positive Control
Included. (Prod. No. ALX-840-030)
Stability
Stable at -80°C up to 1 year, at 4°C up to 3 months.
Dilution
Immunocytochemistry (0.1-1μg/ml) Western Blot (1μg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Reconstitution
Reconstitute with 1ml water (15 minutes at room temperature) .
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Specificity
Recognizes the C-terminus of the EGF receptor (aa 1165-1186) . Recognition is independent of phosphorylation of Tyr 1173 .
Formulation
Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Warranty
This antibody is covered by our Worry-Free Guarantee .
Isotype
IgG1
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