DPPIV/CD26 Assay kit for biological samples

Product Specifications

Product Name Alternative

CD26, Dipeptidyl Peptidase IV, DPP4

Certification

RUO

UniProt

P27487

Applications

Activity assay, Colorimetric detection, Fluorescent detection, HTS

Sensitivity

Can detect DPPIV activity in most or all biological samples.

Stability

Substrates and inhibitor are in DMSO and are stable for up to 6 months. DPPIV enzyme activity decreases with time, even at -80°C.

Compatibility

This product is compatible with the Absorbance 96 Plate Reader .

Shipping Conditions

Dry Ice

Storage Conditions

-80°C

Applications Notes

Correlation of DPPIV activity with disease states, or determination of the efficacy of DPPIV inhibitors administered in vivo .

DPPIV enzyme (Human, recombinant) (Prod. No. BML-SE434-9091) (5 mU; One U=1μmole/min at 37°C, 100μM H-Gly-Pro-pNA. Purity ≥95% (SDS-Page) Storage: -80°C; avoid freeze thaw cycles pNA substrate (H-Gly-Pro-pNA) (Prod. No. BML-P188-9091) (55μl; 100mM in DMSO) Storage: -80°C pNA calibration standard (p-nitroaniline) (Prod. No. BML-KI577) (600μl; 80μM in 50mM Glycine, pH 8.7, 1mM EDTA) Storage: -80°C AMC substrate (H-Gly-Pro-AMC) (Prod. No. BML-P189-9091) (45μl; 50mM in DMSO), Storage: -80°C AMC calibration standard (7-amino-4-methylcoumarin) (Prod. No. BML-KI578) (600μl; 50μM in 50mM Glycine, pH 8.7, 1mM EDTA) Storage: -80°C Inhibitor P32/98 (Prod. No. BML-PI142-9090) (20μl; 1mM in DMSO) Storage: -80°C Assay buffer (Prod. No. BML-KI576) (20ml; 50mM Glycine, pH 8.7, 1mM EDTA; liquid in screw-cap plastic bottle) Storage: -80°C 1⁄2-volume clear microplate (Prod. No. BML-KI101) Storage: Ambient 1⁄2-volume NBS white microplate (Prod. No. BML-KI571) Storage: Ambient