DPPIV (human), (recombinant)
Product Specifications
Specifications
One unit produces 1μmole free pNA per minute, using as substrate 100 μM H-Gly-Pro-pNA (Prod. No. BML-P188) in 50mM TRIS pH 7.5 at 37°C.
Product Name Alternative
Dipeptidyl Peptidase IV, DPP4, CD26
Certification
RUO
UniProt
P27487
Source
Produced in Sf9 insect cells using baculovirus expression system. Active recombinant human DPPIV (aa 29-766) . This represents a naturally-occurring cleaved (soluble) form of DPPIV.
Purification
Purified by multi-step chromatography.
Stability
The enzyme is stable on ice for at least several hours. However, it is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay. After initial defrost, the remaining, undiluted and unused enzyme should be refrozen quickly by, for example, snap-freezing in a dry ice ethanol bath or liquid nitrogen. Aliquot product shall be stored -80°C. Avoid repeated freeze/defrost cycles.NOTE: When stored under the above conditions, this enzyme is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution of the enzyme followed by refreezing could lead to loss of activity.
Purity
≥95% (SDS-PAGE)
Molecular Weight
85.4 kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 10 mM TRIS, pH 8.0, 100mM NaCl.
Applications Notes
Useful tool to study enzyme kinetics, cleave target substrates, and screen for inhibitors.
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