DLL1 (soluble) (human), (recombinant)
Product Specifications
Product Name Alternative
Delta-like protein 1, Delta1
Certification
RUO
UniProt
O00548
Source
Produced in HEK 293 cells. Recombinant human sDLL-1 contains 522 amino-acid residues.
Stability
The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted sDLL-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C.
Endotoxin
< 0.1 ng per μg of sDLL-1.
Purity
≥95% (SDS-PAGE and HPLC)
Activity
Determined by the dose dependent growth suppression of the human acute monocytic leukemia cell line, THP-1. sDLL-1 inhibits the proliferation in THP-1 cells using a concentration of 3-5 μg/ml.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water at a concentration of 0.1 – 1mg/ml. Do not vortex. For long term storage, it is recommended to dilute further in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Molecular Weight
~57-60kDa
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Formulation
Lyophilized from 1X PBS, pH 7.5.
GenBank Number
28514
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