Dkk-1 (mouse), (recombinant) (active)
Product Specifications
Product Name Alternative
Dickkopf-related protein-1
Certification
RUO
UniProt
O54908
Source
Produced in insect cells. Produced in a baculovirus expression system.
Sequence
Mouse Dkk-1 amino acids 33-272, as well as a TEV recognition site and 6 histidines at the C terminus.
Stability
Stable for at least 6 months after receipt when stored as supplied at -80°C.
Endotoxin
<1.0EU/μg (LAL method)
Purity
≥80% (SDS-PAGE)
Activity
Determined measuring the ability to inhibit the induction of the LEADING LIGHT ® Wnt Reporter Cell Line (Prod. No. ENZ-61002) induced for at least 6 hours with 200 ng/ml Wnt3a (Prod. No. ENZ-60001) . The EC50 of this inhibition is 45-55 ng/ml.
Reconstitution
Reconstitute by adding 1.5ml water to make a 3.3μg/ml solution.
Molecular Weight
~40kDa (SDS-PAGE)
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Lyophilized from a solution containing 1X PBS, 1% CHAPS, and 0.1% BSA.
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