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Dkk-1 (mouse), (recombinant) (active)

Product Specifications

Product Name Alternative

Dickkopf-related protein-1

Certification

RUO

UniProt

O54908

Source

Produced in insect cells. Produced in a baculovirus expression system.

Sequence

Mouse Dkk-1 amino acids 33-272, as well as a TEV recognition site and 6 histidines at the C terminus.

Stability

Stable for at least 6 months after receipt when stored as supplied at -80°C.

Endotoxin

<1.0EU/μg (LAL method)

Purity

≥80% (SDS-PAGE)

Activity

Determined measuring the ability to inhibit the induction of the LEADING LIGHT ® Wnt Reporter Cell Line (Prod. No. ENZ-61002) induced for at least 6 hours with 200 ng/ml Wnt3a (Prod. No. ENZ-60001) . The EC50 of this inhibition is 45-55 ng/ml.

Reconstitution

Reconstitute by adding 1.5ml water to make a 3.3μg/ml solution.

Molecular Weight

~40kDa (SDS-PAGE)

Shipping Conditions

Dry Ice

Storage Conditions

-80°C

Formulation

Lyophilized from a solution containing 1X PBS, 1% CHAPS, and 0.1% BSA.

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