CYTO-ID®Autophagy detection kit 2.0

Product Specifications

Certification

RUO

Applications

Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS

Stability

With proper storage, the kit components are stable for one year from date of receipt.

Shipping Conditions

Blue Ice

Storage Conditions

-80°C

Notes

The CYTO-ID ® Autophagy Detection kit 2.0 is a member of the CELLESTIAL ® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications.

Applications Notes

The CYTO-ID ® Autophagy detection kit 2.0 provides a rapid, specific, and quantitative approach for monitoring autophagy in live cells by fluorescence microscopy, flow cytometry, and microplate reader.

Quality Control

Microscopy: When CYTO-ID® Green autophagy detection reagent 2 is incorporated into cells, the accumulation of this fluorescent probe is typically observed in spherical vacuoles in the perinuclear region of the cell, in foci distributed throughout the cytoplasm, or in both locations, depending upon the cell type under investigation. The un-induced cells show very low background staining comparing to autophagic samples prepared as described in the user manual.

CYTO-ID ® Green Detection Reagent 2 Hoechst 33342 Nuclear Stain Autophagy Inducer (Rapamycin) Chloroquine Control 10X Assay Buffer