CYTO-ID® Autophagy detection kit

The CYTO-ID ® Autophagy Detection kit is a member of the CELLESTIAL ® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. Featured in: Genetic Engineering & Biotechnology News – HTS Profiling Method for Autophagy-Modulators Application Notes: Autophagy Analysis Using Object Spot Counting Using Gen5 to Analyze the Size and Number of Autophagosomes Per Nuclei Towards Understanding the Molecular Basis of Parkinson’s Disease: Cell-based Model of Mitophagy and Aggresome Accumulation Response Profiles of Known Autophagy-Modulators Across Multiple Cell Lines: Using CYTO-ID® Autophagy Dye to assess Compound Activity and Toxicity Cell-Based Screening of Focused Bioactive Compound Libraries: Assessing Small Molecule Modulators of the Canonical Wnt Signaling and Autophagy-Lysosome Pathways A Novel Image-Based Cytometry Method for Autophagy Detection in Living Cells Predictive High-Content/High-Throughput Assays for Hepatotoxicity Using Induced Pluripotent Stem Cell (iPSC) -Derived Hepatocytes Visualizing subcellular vesicles to quantitate autophagy in neuronal cells Cited samples: For an overview on cited samples please click here.

The CYTO-ID ® Autophagy detection kit provides a rapid, specific and quantitative approach for monitoring autophagy in live cells by fluorescence microscopy, flow cytometry, and microplate reader.

A sample from each lot of CYTO-ID ® Autophagy detection kit is used to stain HeLa Cells as described in user manual. CYTO-ID ® autophagy detection reagent is incorporated into induced cells, observed as accumulative typical spherical vacuoles in foci or throughout cytoplasm. Comparing to untreated HeLa cells, treated sample demonstrate significant increase in fluorescence under microscope.