Control:Fc fusion protein (human), (recombinant)
Product Specifications
Product Name Alternative
Thy-1 (RLE mutant)
Certification
RUO
Source
Produced in HEK 293 cells. The extracellular domain of mutated hThy-1, hThy-1 (RLE) (aa 20-130), is fused to the Fc portion of human IgG1. The integrin binding sequence RLD of hThy-1 has been changed to RLE abolishing the binding of integrins.
Purification
Purified by multi-step chromatography.
Stability
Stable for at least 6 months after receipt when stored as recommended.
Purity
≥90% (SDS-PAGE)
Reconstitution
Reconstitute with 50μl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Molecular Weight
~ 53 kDa
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Specificity
Does not bind to β3-integrin nor to any of a large number of tested TNF-superfamily members including rhsTRAIL, rhsCD40L, rhsAPRIL, etc.
Formulation
Lyophilized. Contains PBS.
Applications Notes
Control compound for Fc Tagged Proteins, ELISA, Flow Cytometry, Functional Application (serves as a negative control in functional assays of TNF-superfamily members) .
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