COMP (rat) :Angiopoietin-1 (human), (recombinant)
Product Specifications
Product Name Alternative
COMP:Ang-1
Certification
RUO
UniProt
Q15389 (human Angiopoietin-1), P35444 (rat COMP)
Source
Produced in CHO cells. The fibrinogen-like domain (aa 284-498) of human Ang-1 (angiopoietin-1) is fused at the N-terminus to the coiled-coil domain of rat COMP (cartilage oligomeric matrix protein) and to a FLAG®-tag.
Stability
Working aliquots are stable for up to 3 months when stored at -80°C.
Concentration
~0.2-0.4 mg/mL (Please refer to Certificate of Analysis for lot-specific concentration.)
Endotoxin
<0.1EU/μg protein (LAL test; BioWhittaker)
Purity
≥90% (SDS-PAGE)
Activity
Induces phosphorylation of Tie-2 in primary cultured human and mouse vascular endothelial cells. More potent than native Ang-1.
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Notes
FLAG is a registered trademark of Sigma-Aldrich Co.
Specificity
Binds to human Tie-2.
Formulation
Liquid. 0.2μm-filtered solution in PBS containing 0.01% CHAPS.
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