COLOR DE LYS® HDAC colorimetric activity assay kit

Store all components except the microtiter plate at -80°C for the highest stability. The HeLa Nuclear Extract must be handled with particular care in order to retain maximum enzymatic activity. Defrost it quickly in a RT water bath or by rubbing between fingers, then immediately store on an ice bath. The remaining unused extract should be refrozen quickly, by placing at -80°C. If possible, snap freeze in liquid nitrogen or a dry ice/ethanol bath. To minimize the number of freeze/thaw cycles, aliquot the extract into separate tubes and store at -80°C. The COLOR DE LYS® Substrate, when diluted in Assay Buffer, may precipitate after freezing and thawing. It is best, therefore, to dilute only the amount needed to perform the assays of that day.

Nuclear Extracts from HeLa Cells (human cervical cancer cell line) (Prod. No. BML-KI137) (500 μl) Storage: avoid freeze/thaw cycles! COLOR DE LYS ® Substrate (Prod. No. BML-KI138) (50 μL; 50mM in DMSO) Storage: -80°C COLOR DE LYS ® Developer Concentrate (20x) (Prod. No. BML-KI139) (100 μl; 20x stock solution, dilute in HDAC assay buffer before use) Storage: -80°C Trichostatin A (HDAC Inhibitor) (Prod. No. BML-GR309-9090) (100 μl; 0.2mM in DMSO) Storage: -80°C COLOR DE LYS ® Deacetylated Standard (Prod. No. BML-KI141) (30 μl; 10mM in DMSO) Storage: -80°C HDAC Assay Buffer (Prod. No. BML-KI143) (50mM TRIS-HCl, pH 8.0, 137mM NaCl, 2.7 mM KCl, 1mM MgCl 2) (20 ml) Storage: -20°C 1/2 volume microplate (Prod. No. BML-KI101) Storage: Ambient