Cell Counting Kit-F

Principle: The amount of a fluorescent dye, calcein, produced from calcein-AM (3’,6’-Di (O-acetyl) -2’,7’-bis[N, N-bis- (carboxymethyl) aminomethyl]fluorescein . tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Since calcein-AM is highly lipophilic due to the acetoxymethyl groups, it can rapidly permeate into the cytoplasma through the cell membrane. The detection range of the number of viable cells by CCKi-F is from <50 to at least 25,000 cells.