CD44std (human) monoclonal antibody (SFF-304)

Flow Cytometry: Recommended concentration for the unlabelled antibody is 2μg/ml as determined on HACAT cells.Immunohistochemistry (frozen sections, paraffin sections) : Can be used to stain both indirectly or directly acetone-fixed cryostat sections or cell smears. Recommended is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP) or peroxidase anti-peroxidase (PAP) procedures or the three stage immunoperoxidase technique on acetone-fixed cryostat sections. It may be used at a concentration of 2-10μg/ml for cryostat sections. Suitable for paraffin-embedded tissue after microwave treatment.Western Blot: Recommended concentration: 2-10μg/ml.Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.