CD40L, soluble (human) (recombinant) set
Product Specifications
Product Name Alternative
CD154, TNFSF 5
Certification
RUO
UniProt
P29965
Source
Produced in E. coli . The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG ® -tag.
Applications
FUNC
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
CD40L: 0.1 mg/mL after reconstitutionEnhancer: 1 mg/mL
Purity
≥95% (SDS-PAGE) .
Reconstitution
Reconstitute CD40L with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
CD40L (Prod. No. ALX-522-015) and Enhancer for Ligands (Prod. No. ALX-804-034) should be incubated together for 30 min. at room temperature prior to cell application. Effects are typically best seen with 0.5-10μg/ml of CD40L together with 1-2μg/ml of enhancer. This may vary by cell type and experimental conditions. FLAG is a registered trademark of Sigma-Aldrich Co.
Specificity
CD40L binds to human CD40.
Formulation
CD40L: Lyophilized. Contains PBS. Enhancer: Liquid. In PBS, pH 7.4, containing 50% glycerol. The formulation contains no antimicrobial preservatives.
Applications Notes
Can be used to activate human B cells and dendritic cells. Stimulates growth of human B cells. For stimulation of mouse cells via CD40 use MAb to CD40 (mouse) (FGK45) (Prod. No. ALX-805-046) .
Contents
10μg of CD40L, Soluble (human) (recombinant) (Prod. No. ALX-522-015) and 2x50μg of Enhancer for Ligands (Prod. No. ALX-804-034), which increases the biological activity of rhsCD40L at least 1’000-fold.
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