CD40L (soluble) (mouse), (recombinant)

Product Specifications

Product Name Alternative

CD154, TNFSF 5, gp39

Certification

RUO

UniProt

P27548

Source

Produced in HEK 293 cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG ® -tag.

Purification

Purified by multi-step chromatography.

Stability

Stable for at least 6 months after receipt when stored at -20°C.

Concentration

0.1 mg/mL after reconstitution.

Endotoxin

<50EU/mg purified protein (LAL test; Associates of Cape Cod) .

Purity

≥90% (SDS-PAGE)

Reconstitution

Reconstitute each tube with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.

Molecular Weight

~26kDa (SDS-PAGE) .

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

Historical data has shown that mouse CD40L stimulates the proliferation of mouse B cells and dendritic cells. The activity of rmsCD40L increases 1’000-fold (stimulation in the ng/ml range) in the presence of a cross-linking enhancer (see Set Prod. No. ALX-850-075) . FLAG is a registered trademark of Sigma-Aldrich Co.