CD40L (soluble) (human), (recombinant)
Product Specifications
Product Name Alternative
CD154, TNFSF 5, gp39
Certification
RUO
UniProt
P29965
Source
Produced in E. coli . The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG ® -tag.
Purification
Purified by Multi-Step Chromatography.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
0.1 mg/mL after reconstitution.
Purity
≥95% (SDS-PAGE)
Reconstitution
Reconstitute each tube with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Molecular Weight
~18kDa (SDS-PAGE) .
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
Historical data has shown that CD40L stimulates growth of B cells. The activity of rhsCD40L increases 1’000-fold (stimulation in the ng/ml range) in the presence of cross-linking enhancer (see Set Prod. No. ALX-850-064) . For stimulation of mouse cells via CD40 use CD40L, Soluble (mouse) (recombinant) (Prod. No. ALX-522-070 or ALX-850-075) . FLAG is a registered trademark of Sigma-Aldrich Co.
Specificity
Binds to human CD40.
Formulation
Lyophilized. Contains PBS.
Applications Notes
ELISA: binds to CD40 receptor at 1-10 ng/ml.
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