CD40 (human) :Fc (human), (recombinant)
Product Specifications
Product Name Alternative
TNFRSF 5
Certification
RUO
UniProt
P25942
Source
Produced in HEK 293 cells. The cysteine-rich region (aa 21-193) of human CD40 is fused to the Fc portion of human IgG1.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
1 mg/mL after reconstitution.
Endotoxin
<0.1EU/μg purified protein (LAL test; Associates of CAPE COD Inc) .
Purity
≥95% (SDS-PAGE)
Reconstitution
Reconstitute with 50μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Molecular Weight
~54kDa (SDS-PAGE) .
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Specificity
Binds human and mouse CD40L (CD154) .
Formulation
Lyophilized. Contains PBS.
Applications Notes
ELISA: binds to CD40L at 1-10 ng/ml. Can be used together with anti-human IgG1 PAb to detect membrane-bound human or mouse CD40L. CD40:Fc should be used at concentrations between 1 and 100μg/ml depending on the level of CD40L surface expression.
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