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CD137 (human) :Fc (human), (recombinant)

Product Specifications

Product Name Alternative

4-1BB, TNFRSF 9

Certification

RUO

UniProt

Q07011

Source

Produced in HEK 293 cells. The cysteine-rich region of human CD137 (4-1BB) (aa 1-186) is fused to the Fc portion of human IgG1.

Stability

Stable for at least 6 months after receipt when stored at -20°C.

Concentration

1 mg/mL after reconstitution.

Endotoxin

<0.1EU/μg purified protein (LAL test; Associates of Cape Cod) .

Purity

≥95% (SDS-PAGE)

Reconstitution

Reconstitute with 50μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.

Molecular Weight

~50kDa (SDS-PAGE) .

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Specificity

Binds human CD137L (4-1BBL) .

Formulation

Lyophilized. Contains PBS.

Applications Notes

ELISA: binds to CD137L at 10-100 ng/ml. Can be used together with anti-human IgG1 PAb to detect membrane-bound human CD137. Functional Application: Induces proliferation of monocytes. Note: Induction of monocyte proliferation by CD137 required immobilization of the CD137 protein, e.g. by coating of the protein to the surface of the tissue culture dishes.

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