CD137 (human) :Fc (human), (recombinant)
Product Specifications
Product Name Alternative
4-1BB, TNFRSF 9
Certification
RUO
UniProt
Q07011
Source
Produced in HEK 293 cells. The cysteine-rich region of human CD137 (4-1BB) (aa 1-186) is fused to the Fc portion of human IgG1.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
1 mg/mL after reconstitution.
Endotoxin
<0.1EU/μg purified protein (LAL test; Associates of Cape Cod) .
Purity
≥95% (SDS-PAGE)
Reconstitution
Reconstitute with 50μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
Molecular Weight
~50kDa (SDS-PAGE) .
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Specificity
Binds human CD137L (4-1BBL) .
Formulation
Lyophilized. Contains PBS.
Applications Notes
ELISA: binds to CD137L at 10-100 ng/ml. Can be used together with anti-human IgG1 PAb to detect membrane-bound human CD137. Functional Application: Induces proliferation of monocytes. Note: Induction of monocyte proliferation by CD137 required immobilization of the CD137 protein, e.g. by coating of the protein to the surface of the tissue culture dishes.
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