Cathepsin S (human), (recombinant)
Product Specifications
Specifications
≥20 U/mg protein. One unit will hydrolyze one μmole Z-Val-Val-Arg-AMC substrate (Prod. No. BML-P199, 40 μM) per minute at 37°C.
Certification
RUO
UniProt
P25774
Source
Produced in insect cells. Recombinant active cathepsin S cloned from human cDNA as full length proenzyme, auto-activated by incubation in pH 4.5 acetate buffer, and purified. Produced in a baculovirus expression system.
Purification
Purified by multi-step chromatography.
Purity
≥95% (SDS-PAGE)
Molecular Weight
26 kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 100 mM MES, pH 6.5, containing 1 mM EDTA, 50 mM L-cysteine, 10 mM DTT, 0.5% Triton ® X-100, 30% glycerol.
Applications Notes
Useful to study enzyme kinetics, cleave target substrates, and screen for inhibitors.
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