Caspase-8 (human), (recombinant) (active)
Product Specifications
Specifications
5’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate IETD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
Product Name Alternative
FLICE, Mch5, MACH
Certification
RUO
UniProt
Q14790
Source
Produced in E. coli . Contains an N-terminal His-tag.
Purity
≥95% (SDS-PAGE)
Reconstitution
Reconstitute to 1U/μl with PBS containing 15% glycerol.
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Lyophilized.
Applications Notes
Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates or as positive control in caspase activity assays. We recommend using 1U per assay for analyzing caspase activity.
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