Caspase-8 (human), (recombinant) (active)

Product Specifications

Specifications

5’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate IETD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.

Product Name Alternative

FLICE, Mch5, MACH

Certification

RUO

UniProt

Q14790

Source

Produced in E. coli . Contains an N-terminal His-tag.

Purity

≥95% (SDS-PAGE)

Reconstitution

Reconstitute to 1U/μl with PBS containing 15% glycerol.

Shipping Conditions

Dry Ice

Storage Conditions

-80°C

Formulation

Lyophilized.

Applications Notes

Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates or as positive control in caspase activity assays. We recommend using 1U per assay for analyzing caspase activity.