Caspase-4 (human), (recombinant) (active)

Product Specifications

Specifications

~5’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.

Product Name Alternative

ICH-2, Protease TX, ICErel-II

Certification

RUO

UniProt

P49662

Source

Produced in E. coli . Contains an N-terminal His-tag.

Purity

≥95% (SDS-PAGE)

Reconstitution

Reconstitute with PBS containing 15% glycerol to 1U/μl.

Shipping Conditions

Dry Ice

Storage Conditions

-80°C

Formulation

Lyophilized.

Applications Notes

Useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.