Caspase-3 fluorometric assay kit
Product Specifications
Product Name Alternative
CPP32, Yama, Apopain
Certification
RUO
UniProt
P42574
Applications
Activity assay, Fluorescent detection
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
DEVD-AFC emits blue light (λmax = 400nm) ; upon cleavage of the substrate by caspase-3 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3 activity. Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays
Contents
25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125μl DEVD-AFC (1mM), 100μl DTT (1M) .
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