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Caspase-3 (human), (recombinant) (active)

Product Specifications

Specifications

≥15,000 U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate DEVD- p NA (Prod. No. ALX-260-033) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.

Product Name Alternative

CPP32, Yama, Apopain

Certification

RUO

UniProt

P42574

Source

Produced in E. coli . Contains an N-terminal His-tag.

Reconstitution

Reconstitute to 1U/μl in water.

Shipping Conditions

Dry Ice

Storage Conditions

-80°C

Formulation

Lyophilized.

Applications Notes

Useful in studying enzyme regulation, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity. For a complete caspase-3 assay protocol, please refer to Caspase-3 Fluorometric or Colorimetric Assay Kits (Prod. No. ALX-850-216 or ALX-850-215) .

Quality Control

Routinely tested for its ability to cleave the caspase-3 substrates Ac-DEVD- p NA (Prod. No. ALX-260-033) and Ac-DEVD-AFC (Prod. No. ALX-260-032) .

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