Caspase-1 (human), (recombinant) (active)
Product Specifications
Specifications
>5’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate YVAD- p NA (p NA: p -nitroaniline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
Product Name Alternative
Interleukin 1β converting enzyme, IL-1β converting enzyme, ICE
Certification
RUO
UniProt
P29466
Source
Produced in E. coli . Contains an N-terminal His-tag.
Reconstitution
Reconstitute to 1U/μl in water
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Lyophilized.
Applications Notes
Useful in studying enzyme regulation and inhibitors, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
Quality Control
Routinely tested for its ability to cleave the caspase-1 colorimetric substrate Ac-YVAD- p NA (Prod. No. ALX-260-026) .
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